Values emanating from element analysis in roe deer teeth from 3 polluted sites and the control locality in the Nízke Tatry National Park (Low Tatra Mountains National Park – NAPANT) are discussed herein. We found the following highest concentrations in the roe deer teeth: As, Ba, Br, Cl and Na in Žiar nad Hronom; Cd, Cu, Hg and Zn in Spiš and Al, Ca, Co, Fe, Mg, Mn, Pb, Rb, Sb and Sr in Orava. Statistically significant difference was established between the concentration of of As, Cd, Co, Cu, Hg, Na, Pb, Rb, Sr and Zn in roe deer teeth from Žiar, Spiš and Orava sites and the control locality of NAPANT. This obtained data is a useful reference point for comparison with future measurements of air pollution in the examined area, whenever hazards due to heavy metal accumulation in the food chain are assessed.