Soils are the physical and biological matrix in which most of the life-sustaining activities of the tree/soil relationship occur, but to date information on how soils affect walnut fruit production is lacking in Albania. We measured the physical and chemical soil characteristics and their effect on root development system as well as the above-ground biomass and tree characteristics in a walnut (Juglans regia L.) plantation of seedling origin located in the north-eastern part of Albania. Soil of this site with moderately fine texture, slightly acid, reasonably well-drained and sufficient amounts of nutrients favoured the growth of walnut. Trees have developed an intricate extensive root system with the taproot penetrating to 3.7 m deep and many lateral roots which are mainly located in the horizon B at depth 60–80 cm. A soil description indicating the favourable characteristics for growing the species is presented.