The field survey was carried out at 6 locations of the Danube Lowland and 7 locations of the East Slovak Lowland. During the research in 2017-2018 4 species of amphibians were recorded in the monitored localities in the Danube region: Hyla arborea, Pelophylax ridibundus, P. lessonae, P. kl. esculentus. In localities in the East Slovak lowland were recorded 5 species: Bombina bombina, Rana dalmatina, Pelophylax ridibundus, P. lessonae, P. kl. esculentus. All detected species of amphibians are protected species according to national law. The Bombina bombina, Hyla arborea, Pelophylax lessonae, Rana dalmatina belong to the species of European interest. Species of national importance include: Pelophylax ridubundus and P. kl.esculentus.